Doctoral study is a significant milestone in the

Doctoral study is a significant milestone in the perpetual journey of education and the best exposure to this would be through a worthy platform to prove the skills and knowledge that I have learnt. This statement is an endeavour to project my credentials as an aspiring researcher, my achievements as a biotechnology graduate, my interest in this doctoral program and my ambitions.I have always been a curious child, seeking reason and logic behind my observations of the world around me, and of the explanations put forth by others. In 5th grade, I won first prize in science exhibition for working model of water cycle, this increased my inclination towards science. My schooling and college education played a pivotal role in shaping my future. I had excellent facilities and faculty, which laid emphasis on fundamentals. This fired my interest in academics and research and thus I have worked consistently towards this goal, with distinctions in my school exams and a Master’s Degree in Biotechnology. Master’s program in Biotechnology has taught me to analyse various biological topics relevant to a problem systematically. The comprehensive integrated 5-year program taught me both theoretical and practical aspects of a variety of life science subjects Genetics, Cell signalling, Mammalian Physiology, Toxicology, Virology, Tissue Culture, Stem Cell, Molecular biology. As you will find in my curriculum vitae, I have tried to keep my research experience diverse and intellect building. In one of the lecture in basic pharmacology and toxicology professor mentioned about ‘bench-to-bedside’- term used to describe the process by which the results of research done in the laboratory are directly used to develop new ways to treat patients. This got deeply rooted in my mind. In 3rd year of my graduate study I worked in R&D department, HPV Vaccine production, Serum Institute of India Ltd., Pune as intern, I learned about producing recombinant protein L1 ( HPV large capsid protein) through yeast expression system. As an intern, I also learned about downstream processing of the protein into final purified product. This became stepping-stone for my master’s thesis to characterize the purified antigen, optimize and select the suitable aluminium adjuvant for formulation of Virus Like Particle (VLP) – based human recombinant HPV bivalent vaccine and to study in-vitro relative potency and stability of the formulated vaccine. To further partake the concept of bench to bedside I joined Advanced Centre for Treatment, Research and Education in Cancer, Mumbai were I was working on Immuno- profiling of oral cancer patient and studying the role of Notch signalling pathway in TCR signalling. The study included flow cytometry, tumor processing, Lymphocyte separation and use of real time PCR

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