Wash is removed with 2ml of PBS for 2 x 1minutes, using a Pasteur pipette. Approximately 500µl of the FITC-Phalloidin/DAPI/ Alexa Fluor 568 goat anti-rabbit combined stain is added onto each coverslip. This is left for an hour in the dark and at room temperature. For the remainder of the protocol, the cells must continue to be kept […]

 article written by M.Granovetter in May-1973. He studies social-network theories and how micro-level social interactions and networking patterns can further be transformed into a bigger-scale interaction or say social-networks. Author tries to prove importance of weak-ties among groups of individuals by analysing structural components. Article starts with the definition of “strength of an interpersonal tie” (Granovetter, 1973, p […]

Examining the importance of bicycle parking facility towards the reduction of theft of bicycle in Karonga. Statement of Problem Karonga district is one of the districts in Malawi where many people do rely on bicycles as their mode of transport. In Karonga, mainly around Karonga main market, more than four thousand registered bicycles are used as taxi (Chairman […]

Intermediate System to Intermediate System is an extensible intra domain routing protocol designed by Digital Equipment Corporation (DEC) as part of DECnet Phase V networks. IS–IS was made a standard routing protocol by the ISO in 1992 for communication between network devices referred to as intermediate systems (Kaur et al, 2014). The purpose of standardizing IS–IS is to […]

The synthesis of cholesterol can be regulated by targeting the enzymes used in these reactions by various methods, however, it is most commonly exerted at the beginning of the pathway at the HMG-CoA reductase step. Evidence of this can be seen by the sterol mediated regulation of transcription. Sterol regulatory element binding protein (SREBP) binds to a short […]

The Purchasing Guide for Hockey Bags A true hockey player understands the importance of having a high quality hockey bag to haul all of their gaming gear. Nothing is more frustrating than owning a bag that is unable to accommodate each piece of equipment and all accessories required to play the game of hockey. Hockey is not a […]

DVB (digital Video Broadcasting) helps MPEG-TS methodology of broadcasting such that videos are encoded in transport streams. Transferring video pictures need to be added in actual time and with a constant rate of display on the way to hold the movement. The PCR is a time referral this is successive broadcast with every program of a transport stream. […]

The compelling and appropriate integration of project clients is getting to be more critical because it allows obliging higher degrees of complexity in all sorts of projects. Agile project management methodologies such asScrum, Lean/Kanban, the Dynamic Systems Development Framework, or Adaptive Project Framework are gaining popularity since they offer a framework for more profound client inclusion in projects.1.This […]

Nure Useinoski Dr. Bhalla BIO 1130 13 October 2010 Osmosis Is Serious Business 1. The extra fertilizer around the roots of the corn created a hypertonic (cell loses water) environment. 2. The excessive fertilizer caused the soil to turn hypertonic to the plant cell. As a result, water diffused from the plant cells into the soil by osmosis […]

The traditional stereotype of business traveler of being male and middle-aged (41 year- old is the average). •Women are becoming much important in business travel; by 2010 25-30% of all business travel will be under taken by women. •Wealthy Individuals/ highly income •Experts, familiar with the standards offered by different airlines, and able – and willing – to […]